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Epicypher欣博盛生物新品 | 核酸提取緩沖液

更新時間：2023-12-08   點擊次數(shù)：336次

Epicypher新品 | 核酸提取緩沖液


CUTANA™ 細胞核提取緩沖液是CUT&RUN和CUT&Tag實驗中，用于從培養(yǎng)的細胞和組織中提取細胞核的必需試劑。這種緩沖液可與多種真核細胞和組織兼容。以下是使用該緩沖液成功進行細胞核分離的細胞和組織類型：

mouse NIH3T3 fibroblast cells

human A549 non-small cell lung cancer (NSCLC) cells

human K562 leukemia cells

human NCI-H1299 non-small cell lung cancer (NSCLS cells)

human bone marrow derived macrophages

human TIG-1 fetal lung cells

human monocyte derived macrophages

human LoVo colorectal cancer cells

human MV-4-11 macrophage cells

human LNCaP prostate carcinoma cells

human SUM149 triple negative breast cancer (TNBC) cells

human renal primary cells

human GM24385 B-lymphocyte (aka HG002) cells

human peripheral blood mononuclear cells (PBMCs)

human MCF7 breast cancer cells

human intestinal tissue

human MDA-MB-231 breast cancer cells

human HEPM embryonic cells

human SK-MEL-2 melanoma cells


 

只需將試劑盒中的Pre-Nuclei Extraction Buffer和亞精胺與蛋白酶抑制劑混合即可完成該試劑的制備。CUTANA™ 細胞核提取緩沖液針對CUT&RUN和CUT&Tag實驗流程進行了優(yōu)化，從而獲得高質(zhì)量的細胞核，助力您的基因組圖譜分析。

 

試劑盒組成

組分

貨號

CUTANA™ Pre-Nuclei Extraction Buffer      

21-1026a     

1 M Spermidine

21-1026b


保存條件

將Pre-Nuclei Extraction Buffer 4oC儲存, 1 M亞精胺-20oC儲存。

自收到之日起可穩(wěn)定儲存6個月。


驗證數(shù)據(jù)

Epicypher新品 | 核酸提取緩沖液

Figure 1: Nuclei Extraction

Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA™ Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.


訂購詳情

貨號

產(chǎn)品名稱

規(guī)格

21-1026

CUTANA™ Nuclei Extraction Buffer

100 Reactions

 



Epicypher熱銷產(chǎn)品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多詳細信息或相關(guān)產(chǎn)品，請聯(lián)系EpiCypher中國授權(quán)代理商-欣博盛生物