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技術(shù)文章您現(xiàn)在的位置:首頁 > 技術(shù)文章 > MITO-ID®線粒體膜電位檢測試劑盒——ENZO熱銷產(chǎn)品

MITO-ID®線粒體膜電位檢測試劑盒——ENZO熱銷產(chǎn)品

更新時間:2023-12-14   點擊次數(shù):346次

線粒體膜電位(Mitochondrial Membrane Potential,MMP)是判定細(xì)胞健康程度、線粒體膜通透性和細(xì)胞凋亡的一個重要指標(biāo),MMP的喪失通常與細(xì)胞凋亡的早期階段有關(guān)。評估線粒體功能狀態(tài)的基于細(xì)胞的檢測方法正在成為闡明線粒體活動在藥物誘導(dǎo)毒性、細(xì)胞凋亡級聯(lián)以及其他細(xì)胞和生化過程中的作用的有用工具。

Enzo Life SciencesMITO-ID® Membrane potential detection kit包含一種雙發(fā)射陽離子染料,用于檢測活細(xì)胞中的線粒體膜電位(MMP)。在有能量或活躍的細(xì)胞中,MITO-ID® 膜電位試劑因其相對負(fù)電荷而在線粒體中迅速聚集成發(fā)橙色熒光的聚合體,而在細(xì)胞質(zhì)中則以發(fā)綠色熒光的單體存在。然而,在MMP受損的細(xì)胞中,MITO-ID®膜電位試劑主要以綠色熒光單體存在于整個細(xì)胞質(zhì)中,在線粒體中不再表現(xiàn)出橙色熒光。

 

作用機(jī)制

該染料的基本化學(xué)結(jié)構(gòu)由高度共軛的部分組成,使正電荷廣泛離域。該染料能夠選擇性地進(jìn)入線粒體,當(dāng)膜電位增加時,它的顏色會從綠色可逆地變?yōu)槌壬p發(fā)射電位探針)。這種光物理特性是由于在膜極化時可逆地形成J-聚集體,導(dǎo)致在490nm處激發(fā)時,發(fā)射光從~530nm轉(zhuǎn)移到590nm。因此,具有低膜電位的線粒體將積累低濃度的染料,并表現(xiàn)出綠色熒光,而更高極化的線粒體將表現(xiàn)出橙色的熒光。

 

MITO-ID®線粒體膜電位檢測試劑盒——ENZO熱銷產(chǎn)品

 MITO-ID® Membrane potential detection kit線粒體膜電位檢測試劑盒

 

產(chǎn)品特點

● 耐光雙發(fā)射染料,能夠根據(jù)線粒體膜電位狀態(tài)發(fā)出綠色或橙色熒光

● JC-1熒光染料靈敏10倍,并具有卓-越的水溶性

● 無需洗滌步驟

● 適用于化學(xué)/環(huán)境毒性篩選

● 適用于高通量應(yīng)用

 

如需購買ENZO產(chǎn)品,或咨詢產(chǎn)品技術(shù)問題,請聯(lián)系ENZO Life Science代理商欣博盛生物


實驗示例

MITO-ID®線粒體膜電位檢測試劑盒——ENZO熱銷產(chǎn)品

1. MITO-ID® Membrane Potential reagentHeLa細(xì)胞的線粒體進(jìn)行染色,并通過熒光顯微鏡進(jìn)行觀察。橙色熒光聚集體定位于線粒體(橙色通道),而綠色熒光單體主要定位于細(xì)胞質(zhì)(FITC通道)。

 

MITO-ID®線粒體膜電位檢測試劑盒——ENZO熱銷產(chǎn)品

2. 對照組和實驗組細(xì)胞的流式細(xì)胞儀分析。未經(jīng)處理的Jurkat細(xì)胞(左)與用1μM CCCP處理15分鐘的Juekat細(xì)胞(右),用MITO-ID® Membrane Potential reagent對細(xì)胞進(jìn)行染色,并使用流式細(xì)胞儀檢測。

 

產(chǎn)品信息

產(chǎn)品貨號

ENZ-51018-0025/ ENZ-51018-K100

產(chǎn)品名稱

MITO-ID® Membrane potential detection kit 

規(guī)格

1*25tests/1*100tests

短期保存

-20°C

長期保存

-80°C

試劑盒組分

MITO-ID® MP Detection Reagent

Necrosis Detection Reagent

CCCP Control

10X Assay Buffer 1

50X Assay Buffer 2

應(yīng)用

Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS

 

部分產(chǎn)品引用文獻(xiàn)

1. Discovery of the 3-Amino-1,2,4-triazine-Based Library as Selective PDK1 Inhibitors with Therapeutic Potential in Highly Aggressive Pancreatic Ductal Adenocarcinoma: D. Carbone, et al.; Int. J. Mol. Sci. 24, 3679 (2023) 

2. Gene signature predicting recurrence in oral squamous cell carcinoma is characterized by increased oxidative phosphorylation: J.K. Noh, et al.; Mol. Oncol. 17, 134 (2023) 

3. Cu(I) and Cu(II) Complexes Based on Lonidamine-Conjugated Ligands Designed to Promote Synergistic Antitumor Effects: F.D. Bello, et al.; Inorg. Chem. 61, 4919 (2022) 

4. Mitochondrial membrane potential-enriched CHO host: a novel and powerful tool for improving biomanufacturing capability: L. Chakrabarti, et al.; MAbs 14, 2020081 (2022)

5. New glycoconjugation strategies for Ruthenium(II) arene complexes via phosphane ligands and assessment of their antiproliferative activity: D. Iacopini, et al.; Bioorg. Chem. 126, 105901 (2022)

6. New tricyclic systems as photosensitizers towards triple negative breast cancer cell: M. Barreca, et al.; Arch. Pharm. Res. 45, 806 (2022) 

7. Role of caspase-8 and/or-9 as biomarkers that can distinguish the potential to cause toxic-and immune related-adverse event, for the progress of acetaminophen-induced liver injury: T. Noda, et al.; Life Sci. 294, 120351 (2022), Application(s): Microplate reader 

8. Unveiling the Potential of Innovative Gold(I) and Silver(I) Selenourea Complexes as Anticancer Agents Targeting TrxR and Cellular Redox Homeostasis: M.D. Franco, et al.; Chemistry 28, e202201898 (2022) 

9. Altered inflammatory response in FMRP-deficient microglia: J.M. Parrott, et al.; iScience 24, 103293 (2021), Application(s): Fluorescence microscopy 

10. ApoE4 impairs neuron-astrocyte coupling of fatty acid metabolism: G. Qi, et al.; Cell. Rep. 34, 108572 (2021), Application(s): Microplate reader 

11. Botrytis cinerea methyl isocitrate lyase mediates oxidative stress tolerance and programmed cell death by modulating cellular succinate levels: L. Oren-Young, et al.; Fungal Genet. Biol. 146, 103484 (2021), Application(s): Conidia (fungus spore); microscopy 

12. Copper (II) complexes containing natural flavonoid pomiferin show considerable in vitro cytotoxicity and anti-inflammatory effects: J. Vanco, et al.; Int. J. Mol. Sci. 22, 7626 (2021), Application(s): Flow cytometry

13. Depletion of mitochondrial components from extracellular vesicles secreted from astrocytes in a mouse model of fragile X syndrome: B.G. Ha, et al.; Int. J. Mol. Sci. 22, 410 (2021), Application(s): Fluorescence microscopy

14. Inhibiting autophagy targets human leukemic stem cells and hypoxic AML blasts by disrupting mitochondrial homeostasis: K.M. Dykstra, et al.; Blood Adv. 5, 2087 (2021)

15. Selective striatal cell loss is ameliorated by regulated autophagy of the cortex: K. Cho & G.W. Kim; Life Sci. 282, 119822 (2021), Application(s): Flow cytometry