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技術(shù)文章您現(xiàn)在的位置:首頁 > 技術(shù)文章 > GeneTex熱銷抗體 — GAPDH抗體原理/介紹

GeneTex熱銷抗體 — GAPDH抗體原理/介紹

更新時間:2024-12-13   點(diǎn)擊次數(shù):108次

GAPDH抗體識別甘油醛-3-磷酸脫氫酶(GAPDH)蛋白,相基因的產(chǎn)物催化碳水化合物代謝中一個重要的能量產(chǎn)生步驟,即在無機(jī)磷酸鹽和煙酰胺腺嘌呤二核苷酸(NAD)存在的情況下,對甘油醛-3-磷酸進(jìn)行可逆的氧化磷酸化。GeneTex熱銷抗體 —— GAPDH抗體(克隆號GT239)助理您的科研實(shí)驗(yàn)!

 

產(chǎn)品詳情

產(chǎn)品名稱GAPDH antibody [GT239]

別名:glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

反應(yīng)種屬:Candida albicans, Chinese Hamster, Dog, Drosophila, E. coli, Hamster, Human, Monkey, Mouse, Pig, Rat, Yeast, Zebrafish

宿主來源:Mouse

實(shí)驗(yàn)應(yīng)用:EMSA, ICC/IF, IHC-P, WB

同種型:IgG2b

免疫原:Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

克隆性:Monoclonal

克隆號:GT239

純化方式:Affinity purified by Protein G.

偶聯(lián):Unconjugated

產(chǎn)品濃度:1 mg/ml (Please refer to the vial label for the specific concentration.)

保存溫度:Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4oC. For long-term storage, aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.

運(yùn)輸溫度:4°C

預(yù)期分子量:36 kDa

產(chǎn)品形式:Liquid

存儲溶液:PBS, no preservative.

 

驗(yàn)證數(shù)據(jù)

GTX627408.png

 WB Image

GAPDH antibody [GT239] detects GAPDH   protein by western blot analysis.

A. 30 μg yeast whole cell   lysate/extract

10% SDS-PAGE

GAPDH antibody [GT239] (GTX627408)   dilution: 1:10000

The HRP-conjugated anti-mouse IgG   antibody (GTX213111-01) was used to detect the primary antibody.

GTX627408(1).png

ICC/IF:

GAPDH antibody [GT239] detects GAPDH   protein at cytoplasm by immunofluorescent analysis.

Sample: HeLa cells were fixed in   ice-cold MeOH for 5 min.

Green: GAPDH stained by GAPDH antibody   [GT239] (GTX627408) diluted at 1:500.

Blue: Hoechst 33342 staining.

GTX627408(2).png

IHC-P:

GAPDH antibody [GT239] detects GAPDH   protein at cytoplasm by immunohistochemical analysis.

Sample: Paraffin-embedded mouse kidney.

GAPDH stained by GAPDH antibody [GT239]   (GTX627408) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH   6.0, 15 min

 

產(chǎn)品部分引用文獻(xiàn)

1.S Shivaprasad et al. Viruses 2024; 16 (5) : 725 CRISPR Screen Reveals PACT as a Pro-Viral Factor for Dengue Viral Replication.

2.X Chen et al. Nature 2024; 628 (8009) : 818-825 Antisense oligonucleotide therapeutic approach for Timothy syndrome.

3.CP Chen et al. Heliyon 2024; 10 (7) : 29055 Synergistic effects of the combination of trametinib and alpelisib in anaplastic thyroid cancer with BRAF and PI3KCA co-mutations.

4.YL Chen et al. Viruses 2024; 16 : 523 Novel Anti-Viral Properties of the Herbal Extract of Davallia mariesii against Influenza A Virus.

 

訂購詳情

貨號

產(chǎn)品名稱

規(guī)格

GTX627408-S

GAPDH antibody

25 μl

GTX627408

100 μl

 

如需了解更多詳細(xì)信息或相關(guān)產(chǎn)品,請聯(lián)系GeneTex中國授權(quán)代理商-欣博盛生物