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更新時(shí)間:2022-01-28
Zeocin是Phleomycin D1的商業(yè)名稱,是從輪枝鏈霉菌Streptomyces CL990分離得到的一種銅螯合的糖肽抗生素,Zeocin通過嵌入DNA使其斷裂而導(dǎo)致細(xì)胞死亡。Zeocin在細(xì)菌、真核微生物、植物細(xì)胞和哺乳動(dòng)物細(xì)胞上均能發(fā)揮活性,用于篩選攜帶Sh ble基因的轉(zhuǎn)染細(xì)胞。
Zeocin是Phleomycin D1的商業(yè)名稱,是從輪枝鏈霉菌Streptomyces CL990分離得到的一種銅螯合的糖肽抗生素,Zeocin通過嵌入DNA使其斷裂而導(dǎo)致細(xì)胞死亡。Zeocin在細(xì)菌、真核微生物、植物細(xì)胞和哺乳動(dòng)物細(xì)胞上均能發(fā)揮活性,用于篩選攜帶Sh ble基因的轉(zhuǎn)染細(xì)胞。
Zeocin化學(xué)特性
CAS號(hào):11006-33-0
分子式:C55H85O21N20S2Cu - HCl
分子量:1525
推薦使用濃度
大腸桿菌
Zeocin抗性的轉(zhuǎn)化子可以在低鹽LB瓊脂糖培養(yǎng)基(yeast extract 5 g/l, Tryptone 10 g/l, NaCl 5 g/l, Agar 15 g/l,pH 7.5),25 ug/ml Zeocin的條件下篩選。invivogen公司的FastMedia™ Zeo培養(yǎng)基可助您得到最佳篩選效果。
哺乳動(dòng)物細(xì)胞
細(xì)胞系 | 種屬 | 組織 | 培養(yǎng)基 | Zeocin(ug/ml) |
B16 | mouse | melanoma | RPMI | 20-250 |
CHO | hamster | ovary | DMEM | 100-500 |
COS | monkey | kidney | DMEM | 100-400 |
293 | human | kidney | DMEM | 100-400 |
Hela | human | uterus | DMEM | 50-100 |
J558L | mouse | melanoma | RPMI | 400 |
MCF-7 | human | Breast adenocarcinoma | DMEM | 100-400 |
MEFs | mouse | embryonic fibroblasts | DMEM | 200-400 |
THP-1 | human | Leukemic monocytes | RPMI | 200 |
質(zhì)量控制:
純度>90%(HPLC)
活性經(jīng)微生物學(xué)實(shí)驗(yàn)鑒定
保存
4°C或-20°C保存1年,避免反復(fù)凍融。
產(chǎn)品信息:
產(chǎn)品名稱 | 產(chǎn)品編號(hào) | 規(guī)格 | 品牌 | 報(bào)價(jià) | 折扣 |
Zeocin™ | ant-zn-1 | 1g (10 x 1 ml) | Invivogen | ¥2,744 | 請(qǐng)?jiān)?/td> |
Zeocin™ | ant-zn-5 | 5g (50 x 1 ml) | Invivogen | ¥10,598 | 請(qǐng)?jiān)?/td> |
Zeocin™ | ant-zn-5b | 5 g (50 ml bottle) | Invivogen | ¥9,884 | 請(qǐng)?jiān)?/td> |
Zeocin™ | ant-zn-1p | 1 g (powder) | Invivogen | ¥2,380 | 請(qǐng)?jiān)?/td> |
Zeocin™ | ant-zn-5p | 5 g (powder) | Invivogen | ¥8,946 | 請(qǐng)?jiān)?/td> |
文獻(xiàn)引用
•2011 - PNAS, 108: 16663 - 16668
CD147 subunit of lactate/H+ symporters MCT1 and hypoxia-inducible MCT4 is critical for energetics and growth of glycolytic tumors.
Le Floch R, Chiche J, et al.
•2011 - Infect. Immun., 79: 1418 - 1427
NOD-like receptor activation by outer membrane vesicles from Vibrio cholerae non-O1 non-O139 strains is modulated by the quorum-sensing regulator HapR.
Bielig H, Rompikuntal PK, et al.
•2012 - PNAS, 109: 78 - 83
Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.
Sandstrom AG, Wikmark Y, et al.
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