Enzo Life Sciences的ROS-ID®Hypoxia/Oxidative stress detection kit專門用于使用熒光顯微鏡或流式細胞術(shù)對活細胞（懸浮和貼壁）中的缺氧和氧化應(yīng)激水平進行功能性檢測。該試劑盒包含能夠檢測缺氧狀態(tài)（紅色）和氧化應(yīng)激水平（綠色）的熒光探針。

檢測缺氧的染料（紅色）利用缺氧細胞中存在的硝基還原酶活性，將硝基基團轉(zhuǎn)化為羥胺（NHOH）和氨基（NH2）并釋放出熒光探針。

氧化應(yīng)激檢測試劑（綠色）是一種非熒光的、可透過細胞的總ROS檢測染料，可與多種活性物質(zhì)直接反應(yīng)?？墒褂门鋫錁藴薀晒馑兀?90/525 nm）和德克薩斯紅（596/670 nm）濾光片的寬場熒光顯微鏡、共聚焦顯微鏡或配備藍色（488 nm）激光的流式細胞儀進行細胞分析。

產(chǎn)品特點

● 高靈敏度和特異性的熒光探針，可用于檢測活細胞的缺氧和氧化應(yīng)激

● 可用于分析貼壁或懸浮細胞系

● 試劑盒內(nèi)包含整套試劑，包括ROS和缺氧誘導劑

實驗示例

圖1. 檢測人HeLa和HL-60細胞中的缺氧和氧化應(yīng)激水平。用缺氧誘導劑（DFO）和ROS誘導劑（pyocyanin）處理細胞。每個象限的數(shù)字反映了細胞（群體）的百分比。結(jié)果表明，缺氧和氧化應(yīng)激染料具有特異性。

圖2.使用不同試劑誘導HeLa細胞發(fā)生缺氧和氧化應(yīng)激反應(yīng)。缺氧探針在缺氧條件下被細胞硝基還原酶轉(zhuǎn)化后觀察到紅色熒光。

圖3.氧化應(yīng)激（A）和缺氧（B）檢測染料的吸收峰和發(fā)射峰分別為504nm/524nm和580nm/595nm。這些染料可以用488nm的氬離子激光器激發(fā)，并在流式細胞儀上的FL1通道（氧化應(yīng)激染料）和FL3通道（缺氧紅染料）中檢測。

產(chǎn)品信息

部分產(chǎn)品引用文獻

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詳情請聯(lián)系Enzo Life Sciences金牌代理——欣博盛生物