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更新時(shí)間:2022-07-12
Biotin-16-Aminoallyl-2'-deoxycytidine-5'-Triphosphate可用于通過PCR、切口平移、使用Klenow(exo-)DNA聚合酶的引物延伸和末端脫氧核苷酸轉(zhuǎn)移酶標(biāo)記的3'端,將生物素?fù)饺隓NA中。可以使用Moloney鼠白血病病毒(M-MLV)逆轉(zhuǎn)錄酶與此dNTP制備生物素化的cDNA。
Biotin-16-Aminoallyl-2'-dCTP - (N-5002)又名Biotin-16-dCTP, Biotin-16-AA-dCTP。
產(chǎn)品描述
Biotin-16-Aminoallyl-2'-deoxycytidine-5'-Triphosphate可用于通過PCR、切口平移、使用Klenow(exo-)DNA聚合酶的引物延伸和末端脫氧核苷酸轉(zhuǎn)移酶標(biāo)記的3'端,將生物素?fù)饺隓NA中??梢允褂肕oloney鼠白血病病毒(M-MLV)逆轉(zhuǎn)錄酶與此dNTP制備生物素化的cDNA。
產(chǎn)品信息
貨號(hào) | N-5002 | 規(guī)格 | 50 nmole,1 µmole |
純度 | ≥90% by AX-HPLC | 保存溫度 | -20℃或以下 |
分子式 | C32H53N8O17P3S (free acid) | 鹽離子形式 | Li+ |
消光系數(shù) | 5,040 Lmol-1cm-1 at 290 nm | 溶液 | H2O |
濃度 | 5 mM | 骨架 | 5'-三磷酸鹽 |
糖類型 | DNA | 核苷酸類別 | 生物素標(biāo)記 |
應(yīng)用 | 適體、表觀遺傳學(xué)/DNA損傷、體外轉(zhuǎn)錄、誘變、光交聯(lián)研究 |
文獻(xiàn)參考:
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